Generator

Part:BBa_K1010001:Design

Designed by: Gousi Li   Group: iGEM13_SCAU-China   (2013-08-22)

RBS (B0034) + NphR (PNP-responsive positive regulator) + Terminator (B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 862
    Illegal XhoI site found at 978
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 270
    Illegal NgoMIV site found at 925
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Position 684 is mutated from A to G to avoid a Pst1 restriction site.

Source

This part comes from a 5-kb EcoRI DNA fragment cloned from Rhodococcus sp. Strain PN1 involved in PNP oxidation.（Masahiro Takeo et al. 2008.）.

References

Takeo, Masahiro, Masumi Murakami, Sanae Niihara, Kenta Yamamoto, Munehiro Nishimura, Dai-ichiro Kato, and Seiji Negoro. "Mechanism of 4-nitrophenol oxidation in Rhodococcus sp. Strain PN1: characterization of the two-component 4-nitrophenol hydroxylase and regulation of its expression." Journal of bacteriology 190, no. 22 (2008): 7367-7374.

Yamamoto, Kenta, Munehiro Nishimura, Dai-ichiro Kato, Masahiro Takeo, and Seiji Negoro. "Identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in Rhodococcus sp. strain PN1." Journal of bioscience and bioengineering 111, no. 6 (2011): 687-694.